Symposium Program

8:00

Spectral Flow Cytometry in Clinical Practice Paul Mead. St Jude Children's Research Hospital

8:30

Prelim Exploration of Spectral Cytometry for Blast Lineage Screening and Myeloid Maturation Graeme Quest. Queen's University - Kingston Health Sciences Centre

9:00

Future Directions - Contemporary Challenges in Clinical Flow Cytometry: Small Samples, Big Data, Little Time Jonathan Brestoff. Washington University School of Medicine

8:00

Trends and challenges of DIC diagnosis Maha Othman. Queen's University

8:30

Personalized Medicine in Thrombosis and Haemostasis David Lillicrap. Queen's University

9:00

Aggregation When Platelet Count is Low | What Do Guidelines Say Cathy Hayward. McMaster University

10:00

Changes Impacting Lab Developed Tests, a Laboratory Perspective Kristi Smock. University of Utah and ARUP Laboratories

10:30

Addressing Laboratory Testing Utilization Challenges: A Key Component of the Crushing Health Human Resources Challenge in Canada Christine Nielsen. Canadian Society for Medical Laboratory Science (CSMLS)

11:00

Using Labs Wisely by CWC Andrea Thoni. Dalhousie University

10:00

Structural Changes to Genes in Hematologic Malignancy Elizabeth McCready. McMaster University

10:30

Molecular Updates in Myeloproliferative Neoplasms Chris Hillis. McMaster University

11:00

TP53 Mutations in Myeloid Malignancies Alexa Siddon.

1:30

Machine learning-based thalassemia prediction: Model development and over one year experience from routine diagnostics Armin P. Piehler1, 2, Celestino Creatore2, 3, Hanne-Torill Mevik2, Gregor Hoermann1, Eyvind W. Axelsen2, 3. 1MLL Munich Leukemia Laboratory, Munich, Germany.2FA¼rst Medical Laboratory, Oslo, Norway.3University of Oslo, Oslo, Norway

1:45

Real World Experience of Optimal Genomic Mapping in Acute and Chronic Myeloid Leukemias: Findings from a Tertiary Canadian Acute Leukemia Referral Center Sila Usta1, 2, Winston Ukpong1, Larissa Liontos2, 3, Rashmi Goswami2, 3, Raheem Peerani2, 3, Kenneth J Craddock2, 3, Adam C Smith2, 3, Tina YuXuan Luo1, Hong Wang4, Hubert Tsui1, 2, 3, 5. 1Biological Sciences Platform, Sunnybrook Research Institute, Toronto, ON, Canada.2Division of Hematological Pathology, Department Laboratory Medicine and Molecular Diagnostics, Precision Diagnostics and Therapeutics Program, Sunnybrook Health Sciences Centre, Toronto, ON, Canada.3Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada.4Molecular Genetics and Cytogenetics Laboratories, Genetics Program, North York General Hospital, Toronto, ON, Canada.5Department of Immunology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada

2:00

Characterization of Non-Deletional Hereditary Persistence of Fetal Hemoglobin Patients Astyia Z. Franken-Golden, Xi Zhang, Aruna Rangan, Jennifer L. Herrick. Mayo Clinic, Rochester, MN, USA

2:15

Expanding the Molecular Diagnostic Landscape of Hemophilia B through Noncanonical Splicing Variants in F9 Huayang Zhang1, Chong Wang1, Meixiu Gu1, Zhimin Meng1, Yichao Guo1, Weitao Zhang1, Beili Wang1，2，3，4, Wei Guo1，2，3，4. 1Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai China.2Department of Laboratory Medicine, Shanghai Geriatric Medical Center, Shanghai China.3Department of Laboratory Medicine, Wusong Branch, Zhongshan Hospital, Fudan University, Shanghai China.4Department of Laboratory Medicine, Xiamen Branch, Zhongshan Hospital, Fudan University, Xiamen China

2:30

White Blood Cell Enumeration and Differential by Flow Cytometry: The ICSH WBC Reference Method Ben Hedley. London Health Sciences Center

3:00

Laboratory Detection of Malaria Michelle Grant1, Richard McCafferty2. 1Geisinger .2St James's Hospital

3:30

Monocyte Morphology and Classification Gina Zini. Universita' Cattolica, Fondazione Policlinico Gemelli

4:30

Charting the Future of Canadian Adult Acute Myeloid Leukemia (AML) Laboratory Testing: A Canadian Leukemia Study Group Current State Mapping of Diagnostic AML Laboratory Practice Tina YuXuan Luo1Sila Usta1, 2, Eric McGinnis3, Etienne Mahe4, Jose-Mario Capo-Chichi 5, 6, Julie Bergeron7, Tanya Gillan8, Hubert Tsui1, 2, 6, 9, 10. 1Biological Sciences Platform, Sunnybrook Research Institute, Toronto, ON, Canada, Toronto, ON, Canada.2Division of Hematological Pathology, Department Laboratory Medicine and Molecular Diagnostics, Precision Diagnostics and Therapeutics Program, Sunnybrook Health Sciences Centre, Toronto, ON, Canada.3Vancouver General Hospital, University of British Columbia Vancouver, BC, Canada.4Alberta Precision Laboratories, Departments of Pathology and Laboratory Medicine and Division of Hematology and Hematological Malignancies, Department of Medicine, Cumming School of Medicine, Calgary, AB, Canada.5Division of Clinical Laboratory Genetics, University Health Network, Toronto, ON, Canada.6Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada.7Service des laboratoires d'hAcmatologie grappe Optilab CHUM, CEMTL installation Maisonneuve-Rosemont, Montreal, ON, Canada.8. Department of Pathology Central Zone, Nova Scotia Health, Dalhousie University, Halifax, NS, Canada.9Department of Immunology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada.10On behalf of the Canadian Leukemia Study Group / Groupe Canadiene Da, Toronto, ON, Canada

4:42

Revolutionizing Acute Leukemia Prognostication: Standardizing Liquid Biopsy for Serial Monitoring and Precision Medicine in Acute Leukemia _ Paridhi1, Noreen Grace George2, Manpreet Kaur2, Sumita Chaudhry3, Bhavika Rishi1, 2, Aroonima Misra1, 2. 1Department of Pathology, ICMR- Centre for Cancer Pathology, New Delhi, India.2ICMR- National Institute of Child Health a Development Research, New Delhi, India.3Department of Hematology, VMMC a Safdarjung Hospital, New Delhi, India

4:54

Artificial Intelligence Use Cases in a Hematology Laboratory Christian Pohlkamp, Sven Maschek, Torsten Haferlach. MLL Munich Leukemia Laboratory, Munich, Germany

5:06

Automation and artificial intelligence for high-throughput chromosome banding analysis with minimized turnaround time Isolde Summerer, Verena MA¼hlbacher, Torsten Haferlach. Munich Leukemia Laboratory, Munich, Germany

5:18

Comprehensive molecular characterization of methionine metabolism across hematological malignancies reveals association with stemness and anti-tumor immunity Zi-jun Xu, Xiang-mei Wen, Ji-chun Ma, Yu Gu, Ye Jin, Pei-hui Xia, Di Yu, Jinga Zhou, Jiang Lin, Jun Qian. Affiliated Peoplea, Zhenjiang, China

4:30

Factor XIII Deficiency: Insights from a Retrospective, Consecutive Case/Sample Cohort Study Mohammed Al Sharif1, 2, Natalie Mathews1, 3, Subia Tasneem1, Karen A. Moffat1, 4, 5, Stephen A. Carlino5Hayward Catherine1, 4, 5. 1Dept. of Pathology a Molecular Medicine, McMaster University, Hamilton, ON, Canada.2Department of Pathology and Laboratory Medicine, King Abdullah Bin Abdulaziz University Hospital, Riyadh Saudi Arabia.3Hematology/Oncology, Centre Hospitalier Universitaire Sainte-Justine, Montreal, QC, Canada.4Department of Medicine, McMaster University, Hamilton, ON, Canada.5Hamilton Health Sciences, Hamilton, ON, Canada

4:42

Implementation of a Fully Automated ADAMTS13 Activity Assay Utilizing Fluorescence Resonance Energy Transfer (FRET) with a Practical Approach to Address High Background Fluorescence Interference Jing Jin1, Lu M Yang2, James L Zehnder2. 1Special Coagulation, Stanford Health Care Stanford, CA.2Department of Pathology, School of Medicine, Stanford University, Stanford, CA

4:54

Adjusting clot time ratio threshold improves MRX PT DOAC assay’s sensitivity to apixaban Siraj Mithoowani1, Liselotte OnelApv2, Brittany M Salter1Karen Moffat1, 3, 4, Stephen Carlino3, Mark Crowther1, 3. 1Department of Medicine, McMaster University, Hamilton, ON, Canada.2Nordic Biomarker, Umea, Sweden.3Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.4Hamilton Regional Laboratory Medicine Program, Hamilton, ON, Canada

5:18

Platelet Function Assessment Using Dilute Whole Blood for Dense Granule ATP Release Measurement Geoffrey D Wool1, Joseph H Cho2, Clarence Chan1, Krzysztof Mikrut3, Jonathan L Miller1. 1University of Chicago, Department of Pathology, Chicago, IL, USA.2Huntington Health, Pasadena, CA, USA.3UChicago Medicine, Clinical Laboratories, Chicago, IL, USA

4:30

An Evidence-based Ordering Algorithm for Appropriate Utilization of Peripheral Blood Flow Cytometry Anna Dar, Adam C Seegmiller. Vanderbilt University Medical Center, Nashville, TN, USA

4:54

Investigating the Relationship Between Red Blood Cell Biomechanical Properties and Anemia in Cancer Patients Undergoing Chemotherapy Deirdre Finnigan1, Regan Bucciol1, Nick Cruickshanks2, Maha Othman1, 3, 4. 1Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.2Department of Public Health Sciences, Queen's University, Kingston, ON, Canada.3School of Baccalaureate Nursing, St. Lawrence College, Kingston, ON, Canada.4Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

5:06

BCL2/TCL1 as a proposed new combination for the determination of MRD in patients with Blastic Plasmacytoid Dendritic Cell Neoplasm Angeliki Kotsiafti1, Karela Christina1, Tsagarakis Nikolaos2, Oudatzis Georgios1, Milaiou Maroula1, Marinakis Theodoros3, Vasileiou Paraskevi4, Paterakis Georgios1. 1Department of Immunology, GNA, Athens, Greece.2Hematology Laboratory, GNA, Athens, Greece.3Hematology Clinic, GNA, Athens, Greece.4Vasileiou, Paraskevi, Flowdiagnosis Cytometry Diagnostic Center, Athens, Greece

5:18

Interpretable artificial intelligence model for the automatic identification of botryoid neutrophils Anna Merino1, Kevin Barrera2, Gonzalo VerdAo1, Paula San JosAc3, Marina VallbAc3, Angel Molina1, JosAc Rodellar2. 1CORE Laboratory. Biochemistry and Molecular Genetics Department. Biomedical Diagnostic Center. Hospital Clinic, Barcelona, Spain.2Technical University of Catalonia, Barcelona, Spain.3Hematology Department. Hospital de la Santa Creu i Sant Pau, Barcelona, Spain

102

Comparison of hematology analyzers based on large numbers: going beyond traditional verification Albert J. de Graaf1, 2, Arjan de Mare1, 3, Shubham Rastogi4, Jennita Slomp1, 5. 1Unilabs Diagnostics B.V., Enschede, Netherlands.2Saxenburgh Medisch Centrum, Hardenberg, Netherlands.3Ziekenhuisgroep Twente, Almelo, Netherlands.4Horiba Medical, Montpellier, France.5Medisch Spectrum Twente, Enschede, Netherlands

104

Medica’s EasyCount Point-of-Care Analyzer Provides Laboratory-Quality Complete Blood Count and Differential Results in Minutes Lindsay A. Goetz, Kam Lin Wong, Patrick B. Conway, Chen Yi, Steven I. Small. Medica Corporation, Bedford, MA, USA

106

Application of the Digital Hematology Analyzer CellaVision as a Screening Tool in Identification of Intracellular Microorganisms Christine W Kahlow1, David Ayaram1, Mini Jacob2, Hung Luu1, Olga Weinberg1, Weina Chen1, Mingyi Chen1. 1UT Southwestern Medical Center, Dallas, TX, USA.2Parkland Health, Dallas, TX, USA

110

Validation of an International Harmonisation Protocol-compliant measurement procedure for reticulocyte counting by pure erythrocyte gating excluding platelet component. Yutaka Nagai1, 2, Tomohiro Takeda1, Tomoko Arai3, Yoko Yatabe3, Hiromitsu Yokota3, Takayuki Mitsuhashi2, 3, Masatoshi Wakui2, 3, Hiromichi Matsushita2, 3, Hiroshi Kondo1. 1Clinical Laboratory Science, Kansai University of Health Sciences, Osaka, Japan.2Department of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan.3Clinical Laboratory, Keio University Hospital, Tokyo, Japan

112

AI-Driven Human-Machine Interface for Early Detection and differentiation of Sepsis, SIRS and Septic shock with Severity Assessment on Hematology Analyzers Sukesh C Nair1, Francoise Durrieu2, Shubham Rastogi3. 1Christian Medical College, Vellore, India.2Bergonie Institute, Bordeaux, France.3HORIBA ABX SAS, Montpellier, France

114

Evaluation of a newly-developed reticulated platelet assay with a cell membrane-impermeable dye using patient samples. Hiroyuki Sato1, Ayaka Mori1, Mika Araki1, Yoko Yatabe1, Tomohiro Takeda2, Yutaka Nagai2, 3, Tomoko Arai1, Hiromitsu Yokota1, Takayuki Mitsuhashi3, Masatoshi Wakui3, Hiromichi Matsushita3. 1Clinical Laboratory, Keio University Hospital, Tokyo, Japan.2Clinical Laboratory Science, Kansai University of Health Sciences, Osaka, Japan.3Department of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan

116

Real world clinical evaluation of a point of care hematology analyzer in an infusion center Ryan C Shean1, 2, Sterling T Bennett3. 1University of Utah Department of Pathology, Salt Lake City, UT, USA.2ARUP Laboratories, Salt Lake City, UT, USA.3Intermountain Medical Center Department of Pathology, Murray, UT, USA

120

Integrating Artificial Intelligence for Enhanced Review and Disease Screening in Hematology Analyzers Yuting Wang, Zhizhi Liu, Bruce Qian. Dymind Biotechnology Co., Ltd., Shenzhen, China

122

A Retrospective Study of Pediatric D-dimer Test Utilization Rabab A. Al Dawood1, 2, Hassan S. Abusabah1, 3, Natalie Mathews1, 4, James Douketis5, Catherine P. Hayward1, 5. 1Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.2Department of Pathology and Laboratory Medicine, King Fahad Specialist Hospital, Dammam, Saudi Arabia.3Department of Pathology and Laboratory Medicine, King Faisal Medical City for Southern Regions, Abha, Saudi Arabia.4Hematology/Oncology, Centre Hospitalier Universitaire Sainte-Justine, MontrAcal, QC, Canada.5Department of Medicine, McMaster University, Hamilton, ON, Canada

124

Erythrocytes and Platelets in Cancer: An Exploratory Study Regan Bucciol1, 2, Alyssa Qian1, Armita Zandi1, Maha Othman1, 3, 4. 1Department of Biomedical and Molecular Sciences, School of Medicine, Queen's University, Kingston, ON, Canada.2Lawrence Bloomberg Faculty of Nursing, University of Toronto, Toronto, ON, Canada.3School of Baccalaureate Nursing, St Lawrence College, Kingston, ON, Canada.4Clinical Pathology, Mansoura University, Mansoura, Egypt

128

Isolated acquired factor VII deficiency: a case series of a rare haematological disease Eva Gijbels1, Fatin Akmal Baharom2, Anna Vantilborgh3, Katrien M.J. Devreese1, 4. 1Coagulation Laboratory, Department of Laboratory Medicine, Ghent University Hospital, Ghent, Belgium.2Haematology Unit, Pathology Department, Hospital Tunku Azizah, Kuala Lumpur, Malaysia.3Department of Hematology, Ghent University Hospital, Ghent, Belgium.4Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium

130

Comparative Analysis of Commercial Factor VIII (FVIII) Deficient Plasmas with and Without von Willebrand Factor for FVIII One-Stage Clotting Assay (OSA) Stephanie Henneberry1, Mushfiqur Rahman1, Amanda Wood1, K. Maria Notini2, Karen M. Black1, Ali Sadeghi-Khomami1. 1Precision BioLogic Inc., Dartmouth, NS, Canada.2Duke University Medical Center, Durham, NC, USA

134

Reference Values for ST Genesia: Analysis in a Portuguese Population with STG-BleedScreen, STG-DrugScreen, and STG-ThromboScreen - First Report Tiago R. LuAss, Dalila M. PatrAscio, Joana R. Pinheiro, Ana R. Campos, AndrAc G. Coelho, Jorge H. Tomaz. Congenital Coagulopathies Reference Centre-RamA³n Salvado, Thrombosis and Haemostasis Unit, Blood Bank and Transfusion Medicine, ULS Coimbra, Coimbra, Portugal

136

HIT ELISA OD Values Demonstrate Significant Variability Karen A Moser1, 2, Sarah Montgomery3, Kristi J Smock1, 2. 1ARUP Laboratories Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA.2University of Utah Spencer Fox Eccles School of Medicine Department of Pathology, Salt Lake City, UT, USA.3ARUP Laboratories, Salt Lake City, UT, USA

138

Impact of Sodium Citrate Levels in Coagulation Assays: A Controlled ex-vivo study Mushfiqur Rahman, Meghan Bruhm, Karen M. Black, Ali Sadeghi-Khomami . Precision Biologic Inc, Dartmouth, NS, Canada

140

Evaluating the sensitivity of the new fully automated Sysmex HISCL HIT IgG Assay Kit chemiluminescent enzyme immunoassay assay for detecting vaccine induced anti-platelet factor 4 antibodies George Tarpley1, Thomas Pitchford2, Annette Bowyer2, Steve Kitchen2, Kiyoko Kohama3, Akiyo Kawamoto3. 1Sysmex UK Ltd, Milton Keynes, United Kingdom.2Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, United Kingdom.3Sysmex Corporation, Kobe, Japan

142

Follow-up of hemophilia A patients on emicizumab: a comparison between a chromogenic FVIII assay and a modified FVIII one-stage assay. Edwige van der Stegen de Schrieck1, Madeleine Rousseaux1, Catherine Lambert2, Cedric Hermans2, An Van Damme2, Christophe Nougier3Marie-Astrid van Dievoet1. 1Laboratory Department Saint-Luc University Hospital, Brussels Belgium.2Division of Hematology Saint-Luc University Hospital, Brussels Belgium.3Hemostasis and Thrombosis Hospices Civils de Lyon, Lyon France

144

Diagnostic Challenges of Acute Leukemia with Unusual Clinical Presentation and Phenotypic and Genetic Features: A case report Michel Attieh, George Deeb, Saja Asakrah. Department of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA, USA

146

Detection of T cell clonality with 13-color and 15 parameters using flow cytometry - Important tool in the diagnosis of mature T cell neoplasms. Laiz C Bento, Flavia A Sousa, Elizabeth X Souto, Nydia S Bacal. Hospital Israelita Albert Einstein - Citometria de Fluxo, SApo Paulo, Brazil

148

Evaluating T-cell Receptor Isoforms (T-cell receptor constant β chain 1 and T-cell receptor constant β chain 2) by Flow Cytometry in a South African Population. Jamie De Beer, Theo Gerdener. Oncolab Diagnostics, Pretoria, South Africa

150

Down with the tubes, in with the trays: a 96-well tray approach for faster and more efficient diagnostic flow cytometry. Amarilis Figueiredo1, 2, Ian Grace1, Duaa Dabbour1, Evgeniya Shin1, Nour Shurbaji1, Kelly Heinstein1, Robert Liwski1, 2. 1Nova Scotia Health Authority, Halifax, NS, Canada.2Dalhousie University, Halifax, NS, Canada

152

CD11b expression in pediatric B-lymphoblastic leukemia - new insights into immunophenotype and therapy resistance. Rachel Mariani1, 2, Kristian Schafernak1, 2, Jennifer Mason1. 1Phoenix Children's Hospital, Division of Pathology and Laboratory Medicine, Phoenix, AZ, USA.2University of Arizona College of Medicine Phoenix, Phoenix, AZ, USA

154

Imaging flow cytometry to detect Howell-Jolly Bodies for quantitative analysis of splenic function Cesar Rueda, Miya Lu, Kristina Hanson, Ellie Savidge, Tammy WardJennifer Korpik, Mary Reynaud, Charles Quinn, Theodosia Kalfa. Erythrocyte Diagnostic Laboratory, Cincinnati Childrena, Cincinnati, OH, USA

156

Impact of Folate Deficiency on Leukocyte and Platelet Parameters: A Comparative Analysis with Iron and Vitamin B12 Deficiencies Muhammad Shariq Shaikh1, Joao Nunes2, Mari Kono2. 1Department of Pathology a Laboratory Medicine, Aga Khan University Hospital, Karachi, Pakistan.2R a D Centre, Sysmex Asia Pacific, Singapore, Singapore

158

The sensitivity of the DAK-TDT clone aids in the correct evaluation of TdT positivity in hematological neoplasms by flow cytometry in comparison to clone E17-1519 Helena Varela de Araujo, Bruce Loewen, Stefanie Mattson, Bartosz Grzywacz. Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, USA

160

Distribution of PDGFRA gene rearrangement prevalence in clinically suspected case of myeloproliferative disorders with eosinophilia : A Retrospective Cohort Study with Cytogenetic and Mutation Analysis in Major Tertiary Care Hospital in Pakistan Zeeshan Ansar Ahmed , Maria Owais , Muhammad Shariq Shaikh. Aga Khan University Hospital, Karachi, Pakistan

162

Evolving Dynamics of CHIP Variants Post CD19-directed CAR-T therapy in a CLL Patient: A Case Report and Literature Review Wei Du1, Shunsuke Koga1, Gabriel Caponetti1, Jacquelyn J Roth1, Jennifer J.D. Morrissette1, Jakub Svoboda2, Guang Yang1. 1Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.2Division of Hematology Oncology, Abramson Cancer Center, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA

164

Examination of Molecular and Flow Cytometric Findings in relation to Challenging IGHV Mutational Status in Chronic Lymphocytic Leukemia Ibrahim Elsharawi1, 3, Mahboubeh Rahmani1, 3, Sue Robinson4, Tanya Gillan2, 3. 1Nova Scotia Health, Department of Pathology and Laboratory Medicine, Division of Hematological Pathology, Halifax, NS, Canada.2Nova Scotia Health, Department of Pathology and Laboratory Medicine, Molecular diagnostics laboratory, Halifax, NS, Canada.3Dalhousie University, Department of pathology, Halifax, NS, Canada.4Nova Scotia Health, Department of Hematology, Halifax, NS, Canada

166

Comprehensive analysis of clinical, pathological, and molecular features in chronic myelomonocytic leukemia: frequent ASXL1 and NRAS mutations and higher mutation burden in myeloproliferative CMML compared to myelodysplastic CMML Yoonseo Jeong. Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea

168

Clinical Significance of Myelodysplasia-Related Gene Mutations in Myeloid Sarcoma Christine W Kahlow1, Sharon Germans1, Maximiliano Ramia de Cap2, Adam Bagg3, Wei Du3, Olga K Weinberg1. 1University of Texas Southwestern Medical Center, Dallas, TX, USA.2Imperial College Healthcare NHS Trust, London, United Kingdom.3Hospital of the University of Pennsylvania, Philadelphia, PA, USA

170

Screening of TP53-mediated clonal hematopoiesis in patients undergoing therapy with Poly-ADP-ribose polymerase inhibitors Jana Kotaskova1, 2, 3, Lucie Ehrlichova1, Sarka Pavlova1, 2, 3, Eva Zapletalova1, Jitka Malcikova1, 2, 3, Johana Mayerova1, Veronika Navrkalova1, 2, 3, Sarka Pospisilova1, 2, 3, Michael Doubek1, 2, 3, Marketa Bednarikova1. 1Department of Internal Medicine, Hematology and Oncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Brno Czech Republic.2Central European Institute of Technology (CEITEC) Center of Molecular Medicine, Masaryk University, Brno Czech Republic.3Institute of Medical Genetics and Genomics, University Hospital Brno and Faculty of Medicine, Masaryk University, Brno Czech Republic

172

Chromosome aberrations in Plasma Cell Leukemia: A molecular cytogenetic study Dimitrios LiakopoulosStefanos I. Papadhimitriou, Dimitrios Pavlidis, Nikolaos J. Tsagarakis, Ioulia Chaliori, Sofia Chaniotaki, Andria Yiaskouri, Stavros Kalafatis, Athanasios Alexopoulos, Evi Farmaki, Veroniki Komninaka. Hematology Laboratory, GNA 'G. Gennimatas', Athens, Greece

174

De novo acute myeloid leukemia with concurrent clonally dominant MECOM::THADA and ETV6::PDGFRB rearrangements and tyrosine kinase inhibitor resistance Eric McGinnis1, 2, Sina Azad2, Zeid Hamadeh2, Dan Li1, 2, Nadia Medvedev1, 2, Julia Parlow1, Tara Spence1, 2. 1Vancouver General Hospital, Department of Pathology and Laboratory Medicine, Vancouver, BC, Canada.2University of British Columbia, Department of Pathology and Laboratory Medicine, Vancouver, BC, Canada

176

Targeted Gene Expression Profiling for Rapid ABC/GCB Stratification of DLBCL Patients: Evaluating the Prognostic and Predictive Impact of COO Subtyping Veronika Navrkalova1, 2, 3, Andrea Mareckova1, Lenka Radova2, Klara Cinatlova1, Vaclav Kubes4, David Salek1, Andrea Janikova1, Jana Kotaskova1, 2, 3. 1Department of Internal Medicine, Hematology and Oncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Brno, Czech Republic.2Central European Institute of Technology (CEITEC) Center of Molecular Medicine, Masaryk University, Brno, Czech Republic.3Institute of Medical Genetics and Genomics, University Hospital Brno and Faculty of Medicine, Masaryk University, Brno, Czech Republic.4Department of Pathology, University Hospital Brno, Brno, Czech Republic

178

SGPL1 as a Metabolic and Epigenetic Regulator in KMT2A-Rearranged Acute Myeloid Leukemia Zi-jun Xu, Xiang-mei Wen, Yu Gu, Ye Jin, Jiang Lin, Jun Qian. Affiliated Peoplea, Zhenjiang, China

180

B-Cell acute lymphoblastic leukemia with ETV6–ACSL6 fusion and eosinophilia: Complete remission following CAR-T Cell Therapy Alex BarragAin-MuApoz1, Miriam AlbAcniz1, Alejandro Calvera1, Angel Molina BorrAis1, Jordi Esteve2, Anna Merino1. 1CORE Laboratory. Biochemistry and Molecular Genetics Department. Biomedical Diagnostic Center. Hospital Clinic of Barcelona., Barcelona Spain.2Hematology Department, Institute of Cancer and Blood Diseases, Hospital Clinic of Barcelona. IDIBAPS, Barcelona Spain

182

B-lymphoblastic leukemia with Reed-Sternberg-like blast morphology Carley Bekkers1, 2, David Conrad1, 2, 3. 1Dalhousie University, Halifax, NS, Canada.2Nova Scotia Health, Halifax, NS, Canada.3IWK Health, Halifax, NS, Canada

184

Optimization of Colocalized Scoring Method of NAD+ Synthesis Markers in Multiple Myeloma Lara Crone1, Amy M Trottier1, 2, Sam Cutler3, Jeanette E Boudreau1, 4, 5, Mahboubeh Rahmani1, 5, 6. 1Department of Pathology, Dalhousie University, Halifax, NS, Canada.2Division of Hematology, Department of Medicine, Dalhousie University, Halifax, NS, Canada.3Department of Medicine, Dalhousie University, Halifax, NS, Canada.4Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.5Beatrice Hunter Cancer Research Institute, Halifax, NS, Canada.6Division of Hematopathology, Department of Pathology and Laboratory Medicine, Nova Scotia Health Authority, Halifax, NS, Canada

186

Quantification of key diagnostic bone marrow cells using automated digital cytomorphology Richard Dolezilek1David Starostka2, Milos Kudelka3, Eva Kriegova4, Tomas Anlauf3. 1Department of Pathology, Hospital Havirov, Havirov, Czech Republic.2Laboratory of Haematooncology and Clinical biochemistry, Hospital Havirov, Havirov, Czech Republic.3Department of Computer Science, Faculty of Electrical Engineering and Computer Science, Technical University of Ostrava, Ostrava, Czech Republic.4Department of Immunology, Faculty of Medicine and Dentistry, Palacky University and University Hospital Olomouc, Olomouc, Czech Republic

188

A challenging diagnosis of Atypical Chronic Lymphocytic Leukemia Amarilis Figueiredo1, 2, Shannon Murphy1, 2, David Conrad1, 2, 3. 1Dalhousie University, Halifax, NS, Canada.2Nova Scotia Health, Halifax, NS, Canada.3IWK Health, Halifax, NS, Canada

190

A Novel Strategy for Assessing Bone Marrow Plasma Cell Percentage: Development and Internal Validation of a Surrogate Calculation Approach Ethan Gantana1, 2, Zivanai Chapanduka1, 2. 1Stellenbosch University, Cape Town, South Africa.2National Health Laboratory Service, Cape Town, South Africa

192

Generation of an in vitro model of pseudo Pelger-Hüet cells Annelie Mc Corkindale, Veronika Jenei. Scientific and Medical Affairs, Global Marketing, CellaVision AB, Lund, Sweden

194

Automatic Generation of Morphological Descriptions of Peripheral Blood Cells with Fine-tuned Vision-Language Models Laura Ruiz1, Anna Merino3, Kevin Barrera2Jose Rodellar2, Santiago Alferez2. 1Universitat Oberta de Catalunya, Barcelona, Spain.2Department of Mathematics. Technical University of Catalonia, Barcelona, Spain.3CORE Laboratory. Biochemistry and Molecular Genetics Department. CDB., Barcelona, Spain

196

Howell-Jolly-like bodies identified in neutrophils, lymphocytes, and monocytes following CAR T-cell therapy Gabrielle Savoie1, David Conrad1, 2, 3. 1Nova Scotia Health, Antigonish, NS, Canada.2IWK Health, Halifax, NS, Canada.3Dalhousie University, Halifax, NS, Canada

200

Critical misclassifications influence the reliability of automated digital bone marrow cytomorphology David Starostka1, Richard Dolezilek2, Milos Kudelka3, Eva Kriegova4, Tomas Anlauf3. 1Laboratory of Haematooncology and Clinical biochemistry, Hospital Havirov, Havirov, Czech Republic.2Department of Pathology, Hospital Havirov, Havirov, Czech Republic.3Department of Computer Science, Faculty of Electrical Engineering and Computer Science, Technical University of Ostrava, Ostrava, Czech Republic.4Department of Immunology, Faculty of Medicine and Dentistry, Palacky University and University Hospital Olomouc, Olomouc, Czech Republic

202

Howell-Jolly Bodies and Splenic Volume: Risk Factors for Severe Infections in Non-Splenectomized Patients Kaori Uchino1, 2, Yuya Nakagami3, Megumi Enomoto3, Nozomu Shimizu4, Kenichi Kondo4, Takahiro Yamamoto5, Shohei Mizuno1, 2, Kazuhiro Ikegame1, 2, Ichiro Hanamura1, 2, Akiyoshi Takami1, 2. 1Division of Hematology, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute, Japan.2Hematopoietic Cell Transplantation Center, Aichi Medical University Hospital, Nagakute, Japan.3Department of Clinical Laboratory, Aichi Medical University Hospital, Nagakute, Japan.4Central Department of Radiology, Aichi Medical University Hospital, Nagakute, Japan.5Department of Radiology, Aichi Medical University School of Medicine, Nagakute, Japan

204

Use of a simple visual aid improves estimation of red blood cells in a high-power field. Andrew Williams1, David Conrad1, 2, 3. 1Dalhousie University, Halifax, NS, Canada.2IWK Health, Halifax, NS, Canada.3Nova Scotia Health, Halifax, NS, Canada

206

Advancing Hematological Diagnostics: Digital Morphology and Automated Blood Cell Classification in Peripheral Blood and Bone Marrow. Gina Zini1, Patrizia Chiusolo1, 2, Elena Rossi1, 2, Francesco Ramundo2, Nicoletta Pelliccioni2, Valerio De Stefano1, 2. 1UniversitAn Cattolica del Sacro Cuore, Rome .2Fondazione Policlinico Universitario Agostino Gemelli - IRCCS, Rome Italy

210

Reference ranges for percentage maximum aggregation in light transmission platelet aggregometry on automated haemostasis analysers. On behalf of the Autoplate Study Group. Sean Platton1, Fatimot Alaoye2, Peter Baker3, Sarah Harper3, Robert Jones4, Richard Kirby5, Stephen MacDonald6, Aine McCormick7, Danielle White6, Clare Wigley7, Nada Yartey8. 1Haemophilia Centre, Royal London Hospital, Barts Health NHS Trust, London United Kingdom.2Haematology Laboratory, Great Ormond Street Hospital for Children NHS Foundation Trust, London United Kingdom.3Oxford Haemophilia and Thrombosis Centre, Oxford University Hospitals NHS Foundation Trust, Oxford United Kingdom.4Department of Coagulation, Royal Hallamshire Hospital, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield United Kingdom.5Haemophilia Centre, Queena, Nottingham United Kingdom.6Department of Haematology, Specialist Haemostasis Unit, Cambridge University Hospitals NHS Foundation Trust, Cambridge United Kingdom.7Diagnostic Haemostasis and Thrombosis, St Thomasa, London United Kingdom.8National Health Service East and Southeast London Pathology Partnership, London United Kingdom

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Influence of pre-analysis incubation time on light transmission platelet aggregometry on an automated haemostasis analyser. On behalf of the Autoplate Study Group. Sean Platton1, Fatimot Alaoye2, Peter Baker3, Sarah Harper3, Robert Jones4, Richard Kirby5, Stephen MacDonald6, Aine McCormick7, Christopher Reilly-Stitt8, Danielle White6, Clare Wigley7, Nada Yartey9. 1Haemophilia Centre, Royal London Hospital, Barts Health NHS Trust, London United Kingdom.2Haematology Laboratory, Great Ormond Street Hospital for Children NHS Foundation Trust, London United Kingdom.3Oxford Haemophilia and Thrombosis Centre, Oxford University Hospitals NHS Foundation Trust, Oxford United Kingdom.4Department of Coagulation, Royal Hallamshire Hospital, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield United Kingdom.5Haemophilia Centre, Queena, Nottingham United Kingdom.6Department of Haematology, Specialist Haemostasis Unit, Cambridge University Hospitals NHS Foundation Trust, Cambridge United Kingdom.7Diagnostic Haemostasis and Thrombosis, St Thomasa, London United Kingdom.8United Kingdom National External Quality Assurance Scheme for Blood Coagulation, Sheffield United Kingdom.9National Health Service East and Southeast London Pathology Partnership, London United Kingdom

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Masquerading as TTP: A Case of Severe Vitamin B12 Deficiency Presenting as Pseudo-Thrombotic Microangiopathy Eiman A Al Zaabi1, Ravi C Dara1, Hormazdiar D Dastoor2, Rania E Madkour1, Hiba S Al Humaidan3, Chandra M Jha 2. 1Sheikh Shahbout Medical City, Abu Dhabi, United Arab Emirates.2SEHA Kideny Centre, Abu Dhabi, United Arab Emirates.3Dubai Health, Dubai, United Arab Emirates

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Utilization of retuculocyte hemoglobin content in the diagnosis of iron deficiency in dialysis patients Mohammed Almohammadi1, 2, Ammar Sahal1, 3. 1King Saud Bin Abdulaziz University for Health Sciences, Jeddah, Saudi Arabia.2Habib Medical Group (HMG), Jeddah, Saudi Arabia.3King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Jeddah, Saudi Arabia

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Using Machine Learning to Examine Correlations Between Syllectometry Parameters: Insights into Red Blood Cell Biomechanics Baran Dastaran1, Lydia Elbatarny2Maha Othman1, 3. 1Queena, Kingston, ON, Canada.2School of Computing, Queena, Kingston, ON, Canada.3School of Baccalaureate Nursing, St Lawrence College, Kingston, ON, Canada

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iSED PRO: A Rheology-Based Approach to High-Throughput ESR Testing Steve Giarrusso, Megan McCutcheon, Sandra Quaresma, Joseph Langella. ALCOR Scientific, LLC, Smithfield, RI, USA

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Using a Rheology-Based ESR Method to Improve Sample Stability Thomas Koshy2, Joseph Langella1, Megan McCutcheon1, Sandra Quaresma1Steve Giarrusso1. 1ALCOR Scientific, LLC, Smithfield, RI, USA.2Beeatus Solutions, Inc, Palatine, IL, USA

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Sample stability for P50 analysis of Hemoglobin Oxygen Affinity decreases more rapidly at room temperature than at refrigerated temperatures Nicholas Sandercock1, Jessica Graham1, Sheena Sembrano1, Sarah Patterson1, 2. 1Hamilton Regional Laboratory Medicine Program, Hamilton, ON.2Department of Medicine, McMaster University, Hamilton, ON

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Performance Evaluation of Mindray BS-240 Analyzer for Quantitative Analysis of Glucose-6-Phosphate Dehydrogenase Levels Muhammad Shariq Shaikh, Hareem Alam. Aga Khan University Hospital, Karachi, Pakistan

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Defining Laboratory Reference Values for Red Blood Cells’ Rheological Mechanics using Syllectometry Yousra Tera1, 2, Maica Yunon3, Regan Bucciol1, Maha Othman1, 2, 3. 1Department of Biomedical and Molecular Sciences, School of Medicine, Queen's University, Kingston, ON, Canada.2Faculty of Medicine, Mansoura University, Mansoura, Egypt.3School of Baccalaureate Nursing, St Lawrence College, Kingston, ON, Canada

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Comparative study of hematimetric indices during the Dengue epidemic periods of 2015 and 2024 Brendha E Xavier, Erivaldo S JuniorRosana M Cosentino Penteado, Liliana M Suganuma, Andreza O Santos, AndrAca Ap Rocco Villarinho, Laiz C Bento, JoApo C Campos Guerra. Albert Einstein Hospital, SApo Paulo Brazil

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Nucleic Acid Amplification Testing, as a tool of quality assurance in critical patient services. Omama Abbasi, Ayesha Junaid, Khadija Ashraf, Tuba Nadeem. Shifa International Hospital, Islamabad, Pakistan

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Optimizing core hematology laboratory sample disposition and flow using process mining Neal I Callaghan1, Jason Quinn2, Robert Liwski2, Chisholm Natalie2, Allam Shawwa2, Calvino Cheng2. 1Faculty of Medicine, Dalhousie University, Halifax, NS, Canada.2Department of Pathology and Laboratory Medicine, Division of Hematopathology, Halifax, NS, Canada

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Mongolian External Quality Assessment Scheme on Complete Blood Count parameters: A Comprehensive Analysis of Nationwide Proficiency Testing Impact on Inter-laboratory Differences Uranbaigali Enkhbayar1, Gantulga Dashdondov1, Naran Gurjav2, Bolor Altangerel3, Narantuya Namjil3, Delgermurun Altangerel4, Sumiya Dorj4, Uyanga Bayarsaikhan5, Tumendemberel Ochirbat6, Bayarzaya Artbazar7. 1School of medicine, Mongolia-Japan Hospital, Mongolian National University of Medical Science, Ulaanbaatar, Mongolia.2Mongolian Association of Health Laboratory Professions, Ulaanbaatar, Mongolia.3Onoshmed Clinical Laboratory, Mongolia, Ulaanbaatar, Mongolia.4Intermed Hospital, Mongolia, Ulaanbaatar, Mongolia.5SOS Medica International Hospital, Mongolia, Ulaanbaatar, Mongolia.6Sysmex Corporation, Mongolia Representative Office, Ulaanbaatar, Mongolia.7Ministry of Health, Mongolia, Ulaanbaatar, Mongolia

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Selective screening of specimens with markedly elevated INRs can prevent spurious data from entering the electronic medical record Christine Fuja1, Brittany Washburn2, Charles Eby1. 1Washington University School of Medicine in Saint Louis, Saint Louis, MO, USA.2Barnes Jewish Hospital, Saint Louis, MO, USA

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Successful Transition to a Digital Platform for the Institute for Quality Management in Healthcare Morphology Proficiency Testing Program Carlo V. Hojilla1, Anna Johnston2, David Good3, on behalf of the IQMH Hematology Scientific Committee2. 1Department of Pathology a Laboratory Medicine, Mount Sinai Hospital, University of Toronto, Toronto, ON, Canada.2Institute for Quality Management in Healthcare, Toronto, ON, Canada.3Department of Pathology and Molecular Medicine, Queena, Kingston, ON, Canada

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Institute for Quality Management in Healthcare factor assay proficiency testing performance: A 10-year review K A Moffat1S Kulkarni2, A Johnston2, M Sholzberg3, 4, R Selby3, 5, D Good6. 1Hamilton Regional Laboratory Medicine Program and Department of Medicine, McMaster University, Hamilton, ON, Canada.2Institute for Quality Management in Healthcare, Toronto, ON, Canada.3Departments of Medicine and Laboratory Medicine a Pathobiology, University of Toronto, Toronto, ON, Canada.4St. Michaela, Toronto, ON, Canada.5University Health Network and Sunnybrook Health Sciences Centre, Toronto, ON, Canada.6Department of Pathology and Molecular Medicine, Queena, Kingston, ON, Canada.7on behalf of the IQMH Hematology Scientific Committee, Toronto, ON, Canada

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Assessment of the Triage MeterPro D-Dimer Method for use in remote laboratories in Nunavut, Canada W.T. Nishi1, S.A. Carlino2, W.C. Hsu2, K.A. Moffat2. 1Government of Nunavut, Department of Health, Rankin Inlet, NU, Canada.2Hamilton Regional Laboratory Medicine Program, Hamilton, ON, Canada

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Artificial intelligence to interpret interphase fluorescence in situ hybridization results Isolde Summerer, Dennis Bode, Sven Maschek, Christian Pohlkamp, Torsten Haferlach. Munich Leukemia Laboratory, Munich, Germany

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Designing a rational approach for the assessment of peripheral blood lymphocytosis in the acute care setting Charles Yin1, Janika Reyes2, Bryony Walker1. 1Division of Hematological Pathology, Department of Laboratory Medicine and Pathology, Faculty of Medicine a Dentistry, University of Alberta, Edmonton, AB, Canada.2Medical Laboratory Science Program, Faculty of Medicine a Dentistry, University of Alberta, Edmonton, AB, Canada

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Racial Influences on Medical Terminology: A Review of Hematologic Nomenclature Mac Zinck1, Gaynor Watson-Creed1, David Conrad1, 2, 3. 1Dalhousie University, Halifax, NS, Canada.2Nova Scotia Health, Halifax, NS, Canada.3IWK Health, Halifax, NS, Canada