Symposium Program

8:00

New Directions in Anticoagulant Therapy Jeffrey Weitz. Professor of Medicine a Biochemistry, McMaster University

8:30

Pediatric Myeloid Malignancies Jeffery Klco. St Jude Children's Research Hospital

9:00

Equity Issues in Lab Testing Madeleine Verhovsek. McMaster University

10:00

Lupus Anticoagulant Testing in Challenging Situations Katrien Devreese. Ghent University Hospital, Ghent University

10:30

Genetic Testing vs Functional Testing to Diagnose Thrombophilia Juliana Perez Botero. Mayo Clinic

11:00

Novel Anticoagulants (XI and XII Targets) Jean Connors. Brigham and Women's Hospital/Harvard Medical School

10:00

Implementation of TRBC1 in the Assessment of T-cells by Flow Cytometry Katherine Devitt. University of Vermont Medical Center

10:30

Advances in Flow Cytometry for Mixed Phenotype and Ambiguous Leukemias Olga Weinberg. UT Southwestern Medical Center

11:00

Immunophenotyping of Hematopoietic Cell Populations: CLSI H62 Instrument Qualification, Setup, and Standardization Amr Rajab. LifeLabs Medical Laboratory Services

1:30

Berend Houwen Lecture: Systemic Mastocytosis Tracy George. University of Utah/ARUP Laboratories

2:15

Wallace H. Coulter Lecture: Making Molecular Diagnostics Faster. Carl Wittwer. University of Utah / Crestwood Technology

3:45

Role of Third Generation Sequencing in Screening and Diagnosing Hemoglobinopathies Landry Nfonsam. Molecular Genetics Laboratory, Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, Hamilton, Ontario, Canada | Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada

4:15

Handling Common Diagnostic Dilemmas in Hemoglobinopathy Testing Archana Agarwal. University of Utah/ARUP Laboratories

4:45

Genetic Testing in Sickle Cell Disease: From Newborn Screening to Applications for Transfusion Care Yves Pastore. CHU Ste-Justine

3:45

ISCN 2024 Nomenclature for Optical Genome Mapping Jean McGowan-Jordan. Children's Hospital of Eastern Ontario/University of Ottawa

4:15

IGHV Somatic Hypermutation Testing in Chronic Lymphocytic Leukemia: Current Practices, Challenges, and Future Directions Tanya Gillan. Nova Scotia Health/Dalhousie University

4:45

Diagnosis of Hereditary Hematologic Malignancies Amy Trottier. Nova Scotia Health

101

MYELODYSPLASTIC NEOPLASM SCORING FLAG, AN EARLY CLINICAL INDICATOR EVALUATED IN CYTOPENIC PATIENTS USING HORIBA HEMATOLOGY ANALYZER YUMIZEN H2500. Julie BLANCHI1, Sebastien RAIMBAULT2, Benoit RUCHETON1, Marion EVEILLARD3FranAsoise DURRIEU1. 1Institut BergoniAc, Bordeaux, France.2HORIBA ABX SAS, Grabels, France.3University Hospital, Nantes, France

103

Evaluation of MiSelect R System: A Comparison with Flow Cytometry and/or RT-qPCR Assays for Measurable Residual Disease Monitoring in Acute Myeloid Leukemia Ying-Jung Huang1Ju-Yu Tseng2, Ming-Chung Kuo1, 3, Yuen-Chin Wang1, Hsuan-Jen Shih1, Yu-Chieh Tung1, Lee-Yung Shih1, 3. 1Division of Hematology-Oncology, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan.2MiCareo Taiwan Co. Ltd., Taipei, Taiwan.3College of Medicine, Chang Gung University, Taoyuan, Taiwan

105

Performance Evaluation of the PBIA-100A Digital Morphology Analyzer for Platelet Counting Seon Joo Kang, Soyeon Kim, Jeehey Kim, Jin Young Lee, Hee Joo Lee. SD Medical Research Institute, Yongin, South Korea

107

MONOCYTE DISTRIBUTION WIDTH VARIATIONS AND INFLAMMATORY RESPONSE INDUCED BY GRAM-NEGATIVE AND GRAM-POSITIVE LIVE BACTERIA AND THEIR ENDOTOXINS D. Ligi1, C. Della Franca1, F. Salvatori1, S. Ranieri Chiatamone2, G. Brandi3, GF Schiavano4E. Sukhacheva5, F. Mannello1. 1Department of Biomolecular Sciences, Section of Biochemistry and Biotechnology, University of Urbino Carlo Bo, Urbino, Italy.2Operative Unit of Clinical Pathology and Microbiology, Department of Services Teramo, Italy.3Department of Biomolecular Sciences, Section of Pharmacology and Hygiene, University of Urbino Carlo Bo, Urbino, Italy.4Department of Humanities, University of Urbino Carlo Bo, Urbino, Italy.5Beckman Coulter Eurocenter, Nyon, Switzerland

109

The potential utility of extended white blood cell parameters and cell population data in respiratory tract infection diagnosis Tandry Meriyanti1, 2, Chaterine Elisabeth1. 1Siloam Hospitals Lippo Village, Banten, Indonesia.2Pelita Harapan Univerisity, Banten, Indonesia

111

Longitudinal Evaluation of Hematological Values obtained with Reference Automated Hematology Analyzers of Six Manufacturers (2024) - On behalf of The Japanese Society for Laboratory Hematology Standardization Committee (JSLH-SC) - Yutaka Nagai1, 2, Tomohiro Takeda1, Kazuto Tsuruda3, Hiromichi Matsushita2, Tohru Inaba4, Yukako Koike5, Toshiyuki Ikemoto6, Masahiro Jona7, Shin-ichi Fujimaki8, Yoko Yatabe9, Chiaki Ikeda10, Sayaka Mori3, Kazue Yoshitomi11, Ayako Maki12, Kei Shimbo13, Akiyoshi Takami14. 1Kansai University of Health Sciences, Osaka, Japan.2Keio University School of Medicine, Tokyo, Japan.3Nagasaki University Hospital, Nagasaki, Japan.4Kyoto Prefectural University of Medicine, Kyoto, Japan.5Toranomon Hospital, Tokyo, Japan.6Shiga University of Medical Science Hospital, Otsu, Japan.7The University of Tokyo Hospital, Tokyo, Japan.8International University of Health and Welfare, Tochigi, Japan.9Keio University Hospital, Tokyo, Japan.10National Cancer Center Hospital, Tokyo, Japan.11Kindai University Hospital, Osakasayama, Japan.12Osaka Medical and Pharmaceutical University Hospital, Osaka, Japan.13Dokkyo Medical University Hospital, Tochigi, Japan.14Aichi Medical University, Nagakute, Japan

113

Analytical Validation of the iSED® automated analyzer for Erythrocyte Sedimentation Rate (ESR) Joyce Richardson Etokwu, Lukas Graf, Miriam Herma-Looser, Susanne Lutz, Bernadette Landolt, Silke Langhof. Center for Laboratory Medicine, St. Gallen Switzerland

115

Ferritin and Other Laboratory Markers Elevation in Pregnant Patients with Combined COVID-19 and Preeclampsia Versus COVID-19 Alone Dhruv Shah1, Georgiana Nemeti2, 3, Gheorghe Cruciat2, 3, Daniel Muresan2, 3, Chaikh-Sulaiman Mariam-Suzana3, A. Kinga Malinowski4, 5, Rezan Abdul-Kadir6, 7, Maha Othman1, 8, 9 . 1Department of Biomedical and Molecular Medicine, Queen's University, Kingston, ON, Canada.2Obstetrics and Gynaecology I, Mother and Child Department, University of Medicine and Pharmacy of Cluj-Napoca, Cluj-Napoca, Rumania.3Obstetrics and Gynaecology I, County Emergency Hospital Cluj-Napoca, Cluj-Napoca, Rumania.4Department of Obstetrics and Gynaecology, University of Toronto, Toronto, ON, Canada.5Division of Maternal-Fetal Medicine, Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON, Canada.6Department of Obstetrics and Gynaecology, The Royal Free National Health Service Foundation Hospital, London, United Kingdom.7Institute for Women's Health, University College London, London, United Kingdom.8School of Baccalaureate Nursing, St. Lawrence College, Kingston, ON, Canada.9Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

117

Evaluating Unghosted Cells (UGC): An Automated Blood Cell Counter-derived Novel Red Cell Research Parameter for its Clinical Utility and Diagnostic Implications Across a Spectrum of Pathological Conditions Praveen Sharma1, Anita Giri1, Dikshat Gupta2, Minakshi Gupta1, Pulkit Rastogi1, Srinivasan Peyam1, Aashima Arora1, Alka Khadwal1, Elena Sukacheva3, Prashant Sharma1, Reena Das1. 1Postgraduate Institute of Medical Education and Research, Chandigarh, India.2The Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.3Beckman Coulter Eurocenter 22, Nyon, Switzerland

119

A novel method to detect platelet RNA content in platelets using a cell membrane-impermeable dye. Tomohiro Takeda1, Hiromi Uekita1, Risa Hata1, Yutaka Nagai1, 2. 1Clinical Laboratory Science, Kansai University of Health Sciences, Osaka, Japan.2Department of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan

123

Generative AI Revolutionizes Hematology Analysis: Empowering Laboratory and Clinical Diagnostics Yuting Wang1, Guoqing Zhu2, Liyan Cui3, Baohong Yue4, Zhizhi Liu1Bruce Qian1. 1Dymind Biotechnology Co., Ltd., Shenzhen, China.2Institute of Hematology a Blood Diseases Hospital, Tianjin, China.3Peking University Third Hospital, Beijing, China.4The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China

125

Object detection model for quantifying fluorescence in situ hybridization (FISH) signal patterns in chronic lymphocytic leukemia (CLL) Xinjie Xu1, Reid Meyer2, Christopher A Sattler2, Kassandra Fisch2, Ben Black3, Kim Olechovski3, Falon Markow3, Shane Swenson3, Rick Smith3, Jansen Seheult1. 1Division of Hematopathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.2Division of Laboratory Genetics and Genomics, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.3Techcyte, Inc., Orem, UT, USA

127

A Retrospective Study of Adult D-dimer Test Utilization Hassan Abu Sabah1, 2, Rabab Al Dawood1, 3, Natalie Mathews1, 4, James Douketis5, Catherine Hayward1, 5. 1Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.2Department of Pathology and Laboratory Medicine, King Faisal Medical City for Southern Regions, Abha, Saudi Arabia.3Department of Pathology and Laboratory Medicine, King Fahad Specialist Hospital, Dammam, Saudi Arabia.4Hematology/Oncology, Centre Hospitalier Universitaire Sainte-Justine, MontrAcal, QC, Canada.5Department of Medicine, McMaster University, Hamilton, ON, Canada

129

Measurement of Direct Oral anticoagulant (DOAC) and Low Molecular Weight Heparin (LMWH) in plasma containing both, using DOAC-Stop™ and Hepzyme®. Can the use of DOAC-Stop™ and Hepzyme® establish baseline results in these samples. Emma J Broomhead, Jackie Dobson-Storr, Lee J Beckett. Coagulation Department, Northern General Hospital Sheffield, United Kingdom

131

Nordic Biomarker MRX PT DOAC assay – A Comparative Study on Three Coagulation Analysers Jackie Dobson-Storr1, Lee J Beckett1, Emma J Broomhead1, Mark Crowther2, 3, Brittany MA Slater2, Karen Moffatt2, 3, 4, Liselotte Onelov5. 1Coagulation Department, Northern General Hospital, Shefield, United Kingdom.2Department of Medicine, McMaster University, Hamilton, ON, Canada.3Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.4Hamilton Regional Laboratory Medicine Program, Hamilton, ON, Canada.5Nordic Biomarker, Umea, Sweden

133

Evaluation of the Lysis Timer in bleeding disorders of unknown cause Eva Gijbels1, Katrien M.J. Devreese1, 2. 1Coagulation Laboratory, Department of Laboratory Medicine, Ghent University Hospital, Ghent, Belgium.2Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium

135

Distribution Ranges of Apixaban Concentrations for Stroke Prevention in Korean Patients with Atrial Fibrillation Jin-Yeong Han1, Suji Park1, Jae-Ryong Shim1, Kwang-Sook Woo1, Jong-Sung Park2. 1Department of Laboratory Medicine, Dong-A University College of Medicine, Busan, Korea.2Department of Cardiology, Dong-A University College of Medicine, Busan, Korea

137

Development of VerifyNow Quality Control Materials Reflecting Drug Responsiveness to P2Y12 Inhibitors and Aspirin Taekyun Kim1, Miok Choi1, Mijeong Kim1, Miyoung Kim1, 2, Young-Uk Cho1, 2Seongsoo Jang1, 2. 1Department of Laboratory Medicine, Asan Medical Center, Seoul, South Korea.2Departments of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, South Korea

139

Evaluation of APTT mixing test interpretation methods: application of ICSH guidelines and comparison with routine practice Young Kyung Lee1, 2, Youngmin Kim2Jeonghee Lee2. 1Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, South Korea.2Department of Laboratory Medicine, Hallym University Sacred Heart Hospital, Anyang, South Korea

143

Performance of a Novel Bovine Chromogenic Factor VIII Assay in the Recovery of Factor VIII Replacement Therapies Tara Quinton, Karen M. Black, Ali Sadeghi-Khomami. Precision BioLogic Inc., Dartmouth, NS, Canada

145

Data-Driven Establishment of Tube Fill Requirements for PT and PTT on the Automated CS-5100 Coagulation Analyzer Julie R Schmidt, Juan J Sanchez Ramirez, Grace Thorsen, Anna E Merrill. University of Iowa Health Care, Iowa City, IA, USA

147

Clinical Profile of Rare Coagulation Factor Deficiencies in An Asian Population Sim Leng Tien. Singapore General Hospital, Singapore, Singapore

149

Comparison between the Clauss Method and the Calculated Method for Fibrinogen Measurement Paula Torralbo MartAsn, Victor GonzAilvez Agullo, Soledad GarcAsa Chileme, Lara Papay RamAsrez, Jose Vicente Garcia LarioTomas De Haro MuApoz. Hospital Universitario ClAsnico San Cecilio, Granada, Spain

151

Hematological biomarkers useful to monitor thrombotic risk in pregnant patients with Systemic Lupus Erythematosus Rogelio Vega-Agavo1, Miguel A Alvarez-Guerrero2, Yesenia Ambriz-Murillo3, Nallely Garcia-Larragoiti1, Alan F Cano-Mendez1, Sofia Zavala-Guijosa1, Patricia Guzman-Cancino1Martha Eva Viveros-Sandoval1. 1Laboratorio de Hemostasia y Biologia Vascular UMSNH, Morelia, Mexico.2Servicio Reumatologia, Hospital General Dr Miguel Silva, Morelia, Mexico.3Servicio Reumatologia, Hospital de Alta Especialidad ISSSTE, Morelia, Mexico

153

Performance of different Flow Cytometry standardized protocols in detection of B-ALL Measurable Residual Disease Laiz C Bento, Flavia A Sousa, Elizabeth X Souto, Nydia S Bacal. Hospital Israelita Albert Einstein - Citometria de Fluxo, SApo Paulo, Brazil

155

Monitoring Response to Therapy: Expanding marrow assessment beyond residual disease detection Sarah Blain, Fangyan Dai, Chad Hudson, Fan-Chi Hsu, Ashley Eckel, Andrew Menssen, Benjamin Stueben, Laura Pardo, Loren Lott, Keely Ghirardelli, Denise Wells, Michael Loken. HematoLogics, Seattle, WA

157

A single-centre retrospective study investigating the utility and cost of early molecular testing in Monoclonal B-cell Lymphocytosis and Chronic Lymphocytic Leukemia in Southwestern Ontario Ortenc Hoxha1, 2, Mrinal Lad3, Benjamin Hedley2, 3, Ian Chin-Yee1, 2, Cyrus Hsia1, 2, Benjamin Chin-Yee1, 2. 1Division of Hematology, Department of Medicine, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada.2London Health Sciences Centre, London, ON, Canada.33Department of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada

159

Comparative analysis of RAS-associated autoimmune leukoproliferative disorder and RASopathy-associated transient myelomonocytic proliferations – expansion of immunophenotypic and molecular features Rachel Mariani1, 2, Jennifer Mason1, Melissa Stalling3. 1Phoenix Children's Hospital, Division of Pathology and Laboratory Medicine, Phoenix, AZ, USA.2University of Arizona College of Medicine Phoenix, Phoenix, AZ, USA.3Akron Children's Hospital, Akron, OH, USA

161

A multicentre study to evaluate an in-house multiparameter immunophenotypic panel to identify precursor B-cells in the determination of measurable residual disease in paediatric B-cell acute lymphoblastic leukaemia Zanre Nell1, Kennedy N. Otwombe2, Jennifer A. Geel3, Deborah K. Glencross1. 1Department of Haematology and Molecular Medicine, Charlotte Maxeke Johannesburg Academic Hospital, University of the Witwatersrand, Johannesburg South Africa.2Perinatal HIV Research Unit, Chris Hani Baragwanath Hospital, University of the Witwatersrand, Johannesburg South Africa.3Department of Paediatric Oncology, Charlotte Maxeke Johannesburg Academic Hospital, University of the Witwatersrand, Johannesburg South Africa

163

Diagnostic Potential of Q-flag and Q-frags in Beta Thalassemia: A Comparative Analysis with Nutritional Anemias Muhammad Shariq Shaikh1, Joao Nunes2, Mari Kono2. 1Department of Pathology a Laboratory Medicine, Aga Khan University Hospital, Karachi, Pakistan.2R a D Centre, Sysmex Asia Pacific, Singapore, Singapore

165

Diagnosis of Hepatosplenic T Cell Lymphoma directed by Flow CytometryDiagnosis of Hepatosplenic T Cell Lymphoma directed by Flow Cytometry Vanessa Afonso Silva, Daniel Silva Nogueira, Felipe Vieira Rodrigues Maciel, Juliana Carneiro Azevedo, MarAsal Cavalcante de Andrade Silva. University of SApo Paulo, SApo Paulo, Brazil

167

The mutational landscape of classical myeloproliferative neoplasms in the Western Cape Province South Africa Zivanai C Chapanduka1, Marthinus J Dicks1, Ibtisam Abdullah1, 2, Carmen Swanepoel1, Erica-Mari Nell1. 1Stellenbosch University FMHS Division of Haematological Pathology, Cape Town, South Africa.2Division of Haematology Department of Pathology Health New Zealand Northern Region New Zealand, Northern Region, New Zealand

169

IRX1 silencing-mediated drug resistance pathway repression predicts adverse prognosis in FLT3 mutation acute myeloid leukemia Yu Gu1, 2, 4, Jing-Dong Zhou1, 2, Zi-Jun Xu2, 3, Xiang-Mei Wen2, 3, Ting-Juan Zhang1, 2, Jiang Lin2, 3, Jun Qian1, 2. 1Department of Hematology, Affiliated People's Hospital of Jiangsu University, Zhenjiang, China.2Zhenjiang Clinical Research Center of Hematology, Zhenjiang, China.3Laboratory Center, Affiliated People's Hospital of Jiangsu University, Zhenjiang, China.4Department of Hematology and Hematological Laboratory Science, Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China

171

Next-Generation Strategies for Hemophilia A Diagnosis: Lessons learned from 518 Cases from North India Manu Jamwal1, Ritika Sharma1, Hari Kishan Senee1, Narender Kumar1, Heera Singh1, Jasbir Kaur Hira1, Pramod Singh1, Chander Hans1, Deepak Bansal2, Amita Trehan2, Arihant Jain3, Pankaj Malhotra3, Jasmina Ahluwalia1, Reena Das1. 1Department of Hematology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.2Department of Pediatrics (Hematology-Oncology Unit), Postgraduate Institute of Medical Education and Research, Chandigarh, India.3 Department of Clinical Hematology and Medical Oncology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

173

Platelet-VWF Interactions: A Novel Alternative Binding Site for GPIbαGOF on VWF Thomas D. Kazmirchuk1, Calvin Bradbury-Jost1, Anastasiia Koziar1, Jiashu Wang1Maha Othman2, 3, 4, Ashkan Golshani1. 1Carleton University, Ottawa, ON, Canada.2Queen's University, Kingston, ON, Canada.3St. Lawrence College, Kingston, ON, Canada.4Mansoura University, Mansoura, Egypt

175

Tumor-Educated Platelets: Comparison of Platelet RNA Isolation Methods and the Impact of Storage Conditions on Biomarker Detection In-Suk Kim1, Jeong Nyeo Lee2, Dong Uk Kim3. 1Department of Laboratory Medicine, Pusan National University School of Medicine, Pusan National University Yangsan Hospital, Yangsan, South Korea.2Department of Laboratory Medicine, Haeundae Paik Hospital, Inje University College of Medicine, Busan, South Korea.3Department of Internal Medicine, CHA Gumi Medical Center, CHA University School of Medicine, Gumi, South Korea

177

Optimization and proof-of-concept for Oxford Nanopore Technology long-read sequencing with PCR/capture-free adaptive sampling based enrichment in acute myeloid leukemia genomic profiling: a pilot study Eric McGinnis1, 2, Zeid Hamadeh1, 2. 1Vancouver General Hospital, Department of Pathology and Laboratory Medicine, Vancouver, BC, Canada.2University of British Columbia, Department of Pathology and Laboratory Medicine, Vancouver, BC, Canada

179

Development of a simplified and high-sensitive CES assay panel for the genetic classification and risk-stratification in Acute Myeloid Leukemia Yuki Namba, Mayu Ikeda, Rinko Sasaki, Ryotaro Miyamoto, Hikaru Hattori, Motonari Daitho. Sysmex Corporation, Kobe, Japan

183

Molecular Insights into High-Grade B-Cell Lymphomas: A Study from Pakistan Muhammad Shariq Shaikh, Zeeshan Ansar Ahmed. Aga Khan University Hospital, Karachi, Pakistan

185

FOXP1 is associated with oncogenesis and clinical outcomes in hematologic malignancies Xiang-mei Wen1, 2, 3, Zi-jun Xu1, 2, 3, Hao-xi Ni1, 2, 3, Su-wan Liu1, 2, 3, Ye Jin2, 4, Wei Zhao2, 4, Shu-yu Luo1, 2, 3, Yuan-yuan Fang1, 2, 3, Zhen-wei Mao2, Jiang Lin1, 2, 3, Jun Qian2, 4. 1Laboratory Center, Affiliated Peoplea, Zhenjiang, China.2Zhenjiang Clinical Research Center of Hematology, Affiliated Peoplea, Zhenjiang, China.3The Key Lab of Precision Diagnosis and Treatment in Hematologic Malignancies of Zhenjiang City, Affiliated Peoplea, Zhenjiang, China.4Department of Hematology, Affiliated Peoplea, Zhenjiang, China

189

A federated learning approach to generalize blood cell image classification models in multicenter settings Kevin Barrera2, Natali Lorenzo2, Anna Merino1, JosAc Rodellar2. 1CORE Laboratory. Biochemistry and Molecular Genetics Department. Biomedical Diagnostic Center. Hospital Clinic, Barcelona, Spain.2Department of Mathematics. Technical University of Catalonia, Barcelona, Spain

191

A Preliminary Evaluation of a Model for Morphological Recognition of White Blood Cells in Peripheral Blood Smears Using Image Processing Siew Lian Chong1, Asral Wirda Ahmad Asnawi2, Yuen Fen Tan4, 5, Veena Selvaratnam1, Sen Mui Tan1, Chee Onn Leong3. 1Hospital Ampang, Ampang, Selangor, Malaysia.2Sunway Medical Centre, Bandar Baru Sunway, Selangor, Malaysia.3AGTC Genomic Sdn Bhd, Bukit Jalil, Selangor, Malaysia.4Cytovision Sdn Bhd, Kuala Lumpur, Malaysia.5Center for Cancer Research, UTAR, Kajang, Selangor, Malaysia

193

UNRAVELING MONOCYTE AND NEUTROPHIL MORPHOLOGICAL ALTERATIONS IN SEPSIS CONDITION BY DIGITAL MORPHOLOGY AND TRANSMISSION ELECTRON MICROSCOPY C. Della Franca1, S. Ranieri Chiatamone2, M. Battistelli3, F. Salvatori1, D. Ligi1E. Sukhacheva4, F. Mannello1. 1Department of Biomolecular Sciences, Section of Biochemistry and Biotechnology, University of Urbino Carlo Bo, Urbino, Italy.2Operative Unit of Clinical Pathology and Microbiology, Department of Services, qG. Mazziniq Hospital, ASL of Teramo, Teramo, Italy.3Department of Biomolecular Sciences, Section of Morphology, Physiology and Environmental Biology, University of Urbino Carlo Bo, Urbino, Italy.4Beckman Coulter Eurocenter, Nyon, Switzerland

197

Blister Cells in a Sickle Cell Disease Patient with Right Heart Failure, Hyperhemolysis, and Acute Chest Syndrome Amarilis Figueiredo1, Rachelle Blackman1, 2, Kim Ingalls2, Allam Shawwa1, 2. 1Dalhousie University, Halifax, NS, Canada.2NS Health Authority, Halifax, NS, Canada

199

Assessment of ChatGPT analysis of hematopathology images Matt LeRoux1, Elona Turley2, David Conrad1, 3, 4. 1Dalhousie University, Halifax, NS, Canada.2BC Children's Hospital, Vancouver, BC, Canada.3Nova Scotia Health, Halifax, NS, Canada.4IWK Health, Halifax, NS, Canada

201

An Unusual Case of Classic Hodgkin Lymphoma with Strong Expression of B-cell Markers Christopher Liwski1, 2, David Conrad1, 2, 3, Allam Shawwa1, 2, Sorin Selegean1, 2. 1Dalhousie University, Halifax, NS, Canada.2Nova Scotia Health, Halifax, NS, Canada.3IWK Health, Halifax, NS, Canada

203

Discordant plasma cell counts between bone marrow biopsy and aspirate specimens Ross Porter1, Pantelis Andreou1, David Conrad1, 2, 3. 1Dalhousie University, Halifax, NS, Canada.2IWK Health, Halifax, NS, Canada.3Nova Scotia Health, Halifax, NS, Canada

207

Learning from EQA: When is debris not debris? Andrea Teuchert1, Bashori Rahman1, Barbara De la Salle1, Peter Carey2. 1UK NEQAS Haematology, Watford, United Kingdom.2The Newcastle Upon Tyne Hospitals NHS Foundation Trust, Newcastle, United Kingdom

211

Multiple Myeloma Transformation to a Plasmablastic Phenotype: A Diagnostic Dilemma and Therapeutic Challenge Sima Zolfaghari1, Donna Ledingham1, Ronald Angeles2, Muhammad Aslam2. 1Regina General Hospital, Regina, SK, Canada.2Pasqua Hospital, Regina, SK, Canada

215

Assessment of the Stability of Platelet Parameters in Different Anticoagulants Over Time Rosana M Cosentino Penteado, Camila B Monteiro, Cristina M Ito, Glaucia S Gomes, Adriana N Gomes, Zuleide C Dias, Lilian D Reis, Andreza O Santos, Andrea A Rocco Villarinho, JoApo C Campos Guerra. Albert Einstein Hospital, SApo Paulo Brazil

217

Acquisition time for light transmission platelet aggregometry on an automated haemostasis analyser. On behalf of the Autoplate Study Group. Sean Platton1, Fatimot Alaoye2, Peter Baker3, Sarah Harper3, Robert Jones4, Richard Kirby5, Stephen MacDonald6, Aine McCormick7, Danielle White6, Clare Wigley7, Nada Yartey8. 1Haemophilia Centre, Royal London Hospital, Barts Health NHS Trust, London United Kingdom.2Haematology Laboratory, Great Ormond Street Hospital for Children NHS Foundation Trust, London United Kingdom.3Oxford Haemophilia and Thrombosis Centre, Oxford University Hospitals NHS Foundation Trust, Oxford United Kingdom.4Department of Coagulation, Royal Hallamshire Hospital, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield United Kingdom.5Haemophilia Centre, Queena, Nottingham United Kingdom.6Department of Haematology, Specialist Haemostasis Unit, Cambridge University Hospitals NHS Foundation Trust, Cambridge United Kingdom.7Diagnostic Haemostasis and Thrombosis, St Thomasa, London United Kingdom.8National Health Service East and Southeast London Pathology Partnership, London United Kingdom

219

Influence of intra-cuvette stir-speed on light transmission platelet aggregometry on an automated haemostasis analyser. On behalf of the Autoplate Study Group. Sean Platton1, Fatimot Alaoye2, Peter Baker3, Sarah Harper3, Robert Jones4, Richard Kirby5, Stephen MacDonald6, Aine McCormick7, Christopher Reilly-Stitt8, Danielle White6, Clare Wigley7, Nada Yartey9. 1Haemophilia Centre, Royal London Hospital, Barts Health NHS Trust, London United Kingdom.2Haematology Laboratory, Great Ormond Street Hospital for Children NHS Foundation Trust, London United Kingdom.3Oxford Haemophilia and Thrombosis Centre, Oxford University Hospitals NHS Foundation Trust, Oxford United Kingdom.4Department of Coagulation, Royal Hallamshire Hospital, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield United Kingdom.5Haemophilia Centre, Queena, Nottingham United Kingdom.6Department of Haematology, Specialist Haemostasis Unit, Cambridge University Hospitals NHS Foundation Trust, Cambridge United Kingdom.7Diagnostic Haemostasis and Thrombosis, St Thomasa, London United Kingdom.8United Kingdom National External Quality Assurance Scheme for Blood Coagulation, London United Kingdom.9National Health Service East and Southeast London Pathology Partnership, London United Kingdom

221

Prophylactic half- dose platelet transfusion effect on thrombocytopenia, transfusion, and bleeding occurrence Majed Refaai, Christine Cahill, Hannah Spector, Madelyn Gatchell, Bianca Santonastaso. University of Rochester, Rochester, NY, USA

223

Silent platelet clumps? Evaluation of their impact to the platelet count Lihjen Sun, Huguette Mulumba, Christine Rongey. Abbott, Santa Clara, CA, USA

225

From Bone Marrow to Blood: Macrocytic Hemolytic Anemia and Skewed Chimerism Reveal Reconstitution of β-Thalassemia 6 years Post-Hemopoietic Stem Cell Transplant Fares Khaled AlFares1, 2, 3, David Conrad2, 3, 4, Bruce Crooks2, 3. 1Kuwait University Faculty of Medicine, Kuwait City, Kuwait.2Dalhousie University Faculty of Medicine, Halifax, NS, Canada.3IWK Health, Halifax, NS, Canada.4Nova Scotia Health, Halifax, NS, Canada

227

The Clinical Utility of ESR and CRP in Inflammation: Evidence from Literature and Guiding Effective Application Mazen Assar1, 2Maha Othman2, 3, 4. 1Internal Medicine Department, University of British Columbia, Vancouver, BC, Canada.2Department of Biomedical and Molecular Sciences, School of Medicine, Queen's University, Kingston, ON, Canada.3School of Baccalaureate Nursing, St Lawrence College, Kingston, ON, Canada.4Faculty of Medicine, Mansoura University, Mansoura, Egypt

233

Evaluation of a New HbA1c Analyzer for Accurate HbA1c Measurement in Samples Containing Hemoglobin Variants Yueying Huang1, Weijie Xie1, Anping Xu1, Ling Ji1Yan Liu2. 1Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen China.2Clinical Department (IVD), Shenzhen Mindray Bio-Medical Electronics Co., Ltd, Shenzhen China

235

A Family with a Rare Haemoglobin Variant (Hb Port Phillip) Wei Lin Kwan, Zhao Qin Lim, Lai Ngah Leong, Noor Lydia Mohd Saleh, Soh Hong Ang, Joyce Ching Mei Lam. KK Women's and Children's Hospital, Singapore Singapore

241

Evaluation of Diesse Cube 30 Touch Erythrocyte Sedimentation Method with Alcor iSED ESR Analyser H.J. Yeo, F. Ilagan, K.L. Cheng, V. Prasad. Khoo Teck Puat Hospital, Department of Laboratory Medicine, Singapore, Singapore

243

EFLM Biological Variation Estimates of Hematology Data Are Not Fit for Newer Purposes; Let’s Use Real Patient Data George Cembrowski1, 2, Jialin Qiu1, Christopher McCudden3, 4. 1University of Alberta, Edmonton, AB, Canada.2CCQCC, Edmonton, AB, Canada.3University of Ottawa, Ottawa, ON, Canada.4Ottawa Hospital, Ottawa, ON, Canada

245

An Exploratory Quality Analysis of Bone Marrow Aspirate Derived Plasma Processing for Potential Future Clinical and Research Applications In Myeloid Neoplasms Thibika Gunalingam1, 2, 3, Alexandra Misura1, 2, Katarina Czibere1, 2, Winston Ukpong1, 2, 3, Mira Jani1, 3, 4, Tina Khazaee1, 2, Paul Yip2, 5, Signy Chow6, 7Hubert Tsui1, 2, 5, 8, 9. 1Sunnybrook Biobank, Department of Laboratory Medicine and Molecular Diagnostics, Precision Diagnostics and Therapeutics Program, Sunnybrook Health Sciences Centre, Toronto, ON, Canada.2Biological Sciences Platform, Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada.3Arts and Sciences Cooperative Program, University of Toronto a, Toronto, ON, Canada.4Evaluative Clinical Sciences, Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada.5Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada.6Hematology Site Group, Odette Cancer Centre, Sunnybrook Health Sciences Centre, Toronto, ON, Canada.7Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada.8Division of Hematological Pathology, Department of Laboratory Medicine and Molecular Diagnostics, Precision Diagnostics and Therapeutics Program, Sunnybrook Health Sciences Centre and Sunnybrook Research Institute, Toronto, ON, Canada.9Department of Immunology, University of Toronto, Toronto, ON, Canada

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Driving Excellence in Hematology Proficiency Testing: Insights from the 2024 Institute for Quality Management in Healthcare Customer Satisfaction Survey Karen A Moffat1, 2Anna Johnston3, Sumedha Kulkarni3, Tess Koerner4, Brittnee V Ross3, Miranda Wozniak5, David Good6. 1Hamilton Regional Laboratory Medicine Program, Hamilton, ON, Canada.2Department of Medicine, McMaster University, Hamilton, ON, Canada.3Institute for Quality Management in Healthcare, Toronto, ON, Canada.4Accreditation Canada, Ottawa, ON, Canada.5Dynacare Medical Laboratories, Brampton, ON, Canada.6Department of Pathology and Molecular Medicine, Queen's University, Kingston, ON, Canada

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A Reference Range Interval Establishment in a Tertiary Teaching Hospital: Performance Evaluation on Mindray BC6200 Nurul Asyikin Nizam Akbar1, 2, Zefarina Zulkafli1, 2, Ahmad Sharifuddin Mukhtarruddin1, 2, Salfarina Iberahim 1, 2, Nur Ilyia Syazwani Saidin 1, 2, Rosnah Bahar 1, 2, Mohd Nazri Hassan1, 2, Marne Abdullah 1, 2, Razan Hayati Zulkeflee1, 2, Mohamad Nashrul Ghazali3, Muhammad Faiz Mohd Ismail 3. 1Department of Haematology a Transfusion Medicine Unit, School of Medical Sciences, Universiti Sains Malaysia (USM), Kota Bharu, Malaysia.2Hospital Pakar Universiti Sains Malaysia (HPUSM), Kota Bharu, Malaysia.3Utas Maju Sdn Bhd, Petaling Jaya, Malaysia

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Accelerated Processes in Laboratory Hematology with Automation Christopher Yu1, Habib M. Razavi2, 3, Majid Moteabbed2, 3, Richard Yu2. 1Faculty of Health Sciences, Western University, London, ON, Canada.2Division of Hematopathology and Transfusion Medicine, Fraser Health Authority, Surrey, BC, Canada.3Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada